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Abstract Details

Role of NOX2 in the BDNF dysregulation and cognitive deficits after sepsis
Aging, Dementia, and Behavioral Neurology
P6 - Poster Session 6 (12:00 PM-1:00 PM)
10-011
Sepsis has been reported to increase the risk of cognitive impairment. Following systemic inflammation, a corresponding neuroinflammation including glia activation and excessive pro-inflammatory factors are rapidly induced in brain, possibly affecting the crucial brain capability. 

BDNF represents one of the major mediators of neuroplasticity. We wanted to evaluate one of the most interesting hypotheses: NOX2 plays a role in the involvement of the neurotrophic factor BDNF dysregulation and cognitive deficient after sepsis.

Mice with deficits of NOX regulatory subunit/NOX2 organizer p47phox (p47phox-/-) and wild-type (WT) mice were used. Lipopolysaccharide (LPS) intraperitoneal injection was used to induce systemic inflammation. Spatial learning and memory were compared among treatment groups using the radial-arm maze task. Brain tissues were evaluated for the transcript levels of proinflammatory cytokines, while immunofluorescence staining and immunoblotting were used to determine the damaged neurons and the levels of BDNF.

Cognitive impairment following systemic inflammation was significantly attenuated in the p47phox-/- mice compared with the WT mice. We found that the levels of BDNF in astroglia and neurons were significantly reduced within 10 days after the LPS injection in parallel with glia activation and increased cytokines in brains. We further revealed that p47phox deletion could reduce glia activation, BDNF downregulation, and cognitive deficits, compared to those in WT-type mice following LPS injection.

Our results suggest that alteration of BDNF could be related to excessive neuroinflammation, subsequently involved in developing cognitive impairment. NOX2 might play a role in the pathogenesis of the development of cognitive deficits after systemic inflammation in a mouse model. 
Authors/Disclosures
Wan-Yu Huang
PRESENTER
No disclosure on file
No disclosure on file
No disclosure on file
No disclosure on file
No disclosure on file
Hung-Ming Wu No disclosure on file