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Abstract Details

Identification of Cardiovascular Biomarkers in a Rat Model of Obstructive Sleep Apnea
Sleep
P9 - Poster Session 9 (8:00 AM-9:00 AM)
9-003
Obstructive sleep apnea (OSA) has emerged as a recognized risk factor for cardiovascular disease. We investigate miRNA expression patterns and gene expression changes related to cardiovascular disease, shedding light on the neurologic implications of this critical intersection between sleep disorders and cardiovascular health.
Our study aims to identify specific cardiovascular biomarkers associated with OSA using a rat model. 

We induced OSA in a rat model by administering hyaluronic acid-based filler (0.1 mL/100 g body weight) at the base of the tongue. Blood pressure measurements were obtained using the CODA-NIBP system at 22 and 44 weeks post-OSA induction. Polysomnographic assessments were performed to gauge the similarity of the rat model to human OSA. Expression profiles of miRNAs and mRNAs were analyzed using the GeneChip rat gene ST 2.0 and Affymetrix miRNA 4.0 microarrays, respectively.

Our OSA rat model exhibited clinical similarities to human OSA, including elevated apnea-hypopnea and oxygen desaturation indices. We observed increased blood pressure, left ventricular hypertrophy, and systemic arterial changes. Gene expression analysis revealed alterations associated with cardiovascular diseases such as atherosclerosis and myocardial injury. Notably, miRNA profiling identified miR-23a-5p and miR-375-3p linked to endothelial dysfunction, myocardial injury, inflammation, apoptosis, and atherosclerosis.

Our study efficiently replicates the cardiovascular implications of human OSA in a rat model, revealing structural and functional changes within the cardiovascular system. We unveil two significant biomarkers, miR-23a-5p and miR-375-3p, with concurrent increases in both blood and systemic arteries. These findings contribute to our understanding of the pathophysiological mechanisms underlying OSA-related cardiovascular complications, with potential neurologic implications.

Authors/Disclosures
Dong-Ick Shin, MD, PhD (Chungbuk National University)
PRESENTER
Dong-Ick Shin, MD, PhD has nothing to disclose.
Ho-seong Han Ho-seong Han has nothing to disclose.