Abstract Details

Title Is it Time to Pay Attention to Circulating MicroRNAs as a Diagnostic Biomarker in REM Behavior Disorder?

Topic Sleep

Presentation(s) P1 - Poster Session 1 (12:00 PM-1:00 PM)

Poster/Presentation
Number 5-009

Objective To detect the expression of two circulating microRNAs (miRNAs) in patients with prodromal Parkinson's Disease (PD) as potential early diagnostic biomarkers in patients with REM behavior disorder (RBD).

Background Parkinson Disease (PD) is the second most common neurodegenerative disorder worldwide and it is well known that rapid eye movement sleep behavior disorder (RBD) may begin several years to decades before the onset of PD. The analysis of miRNAs as biomarkers for neurodegenerative disorders is a rapidly evolving ?eld and recent evidence suggest that blood circulating miRNAs are promising biomarkers for PD.

Design/Methods Fifteen patients with RBD, ten with PD and fifteen control were recruited from the Department of Neurology, CEMIC, Buenos Aires, Argentina. The patients were clinically examined and evaluated using the Unified Parkinson’s Disease Rating Scale (UPDRS), Hoehn and Yahr Scale, Hong Kong Screening Questionnaire (RBDQ-HKQ), Montreal Cognitive Assessment (MoCa), Cognitive SCOPA (SOCPA-COG), Autonomic SCOPA (SCOPA-AUT), Farnsworth-Munsell 100 Hue Color Vision Test (FM100HCVT), Pittsburgh Sleep Quality Index (PSQI) and Epworth Sleepiness Scale (ESS). All control subjects (C) had no history of neurologic/psychiatric disorders. Plasma samples were collected followed by a real-time PCR-based miRNA screening to detect changes of miRNA expression in our group of patients.

Results

Patients with PD and RBD presented increased values respect to control for PSQI (PD:7.1±2.8, RBD:7.6±4.8, C:4.3±3.7), ESS(PD:6.5±2.3,RBD:7.4±3.2, C:4.1±1.8) and FM100HCVT(PD:165±33, RBD:169±125,C: 86.4±57.8) (p<0.05). The serum levels of miRNA 29a were significantly decreased (PD:0.14±0.4, RBD:0.32±0.4, C:0.61±0.5) and miRNA27 (PD:0.06±0.5, RBD:0.11±0.5, C:0.04±0.7) significantly increased in patients with RBD and PD (Wilcoxon Signed Rank test, p<0.05). A non significant correlation between increased values of FM100HCVT and miRNA levels were found in PD and RBD.

Conclusions

Serum miRNA 29a/27 were respectively downregulated/upregulated in patients with PD and RBD. This study shows that the early detection of altered plasmatic levels of miRNAs can be considered a potential prodromal biomarker in RBD patients.

Authors/Disclosures
Daniela L. Giardino, MD (CEMIC) PRESENTER	No disclosure on file
Arturo Garay, MD (CEMIC)	No disclosure on file
	No disclosure on file
	No disclosure on file
	No disclosure on file
Arturo Garay, MD (CEMIC)	No disclosure on file